Is e teicneòlas a th ’ann an RT-PCR a tha a’ cothlamadh tar-sgrìobhadh cùil RT de RNA agus leudachadh slabhraidh polymerase (PCR) de cDNA.Tha teicneòlas RT-PCR mothachail agus ioma-ghnìomhach, agus faodar a chleachdadh gus ìre faireachdainn gine ann an ceallan / maothran a lorg, susbaint bhìorasan RNA ann an ceallan, agus clonadh dìreach sreathan cDNA de ghinean sònraichte.
Bun-bhuannachd:
✱ Tha seasmhachd làidir aig pasgan RT Foregene aig teòthachd freagairt 50-65 ℃ (faodaidh 65 ° C an dàrna ìre de RNA fhosgladh gus toirt air an ath-sgrìobhadh tar-chuir a dhol air adhart gu rèidh)
✱ Fulangas làidir, is urrainn dha cuid de ethanol agus salann guanidine fhulang
| Fo-Sreath | Ainm toraidh | Sònrachaidhean | Àireamh catalog | Cumhachan stòraidh |
| Sreath PCR measgachadh | 5ml | PH-01013 | -20 ℃ | |
| 40 ml | PH-01015 | |||
| 5ml | PH-01013-B | -20 ℃ | ||
| 40 ml | PH-01015-B | |||
| 2 × Siostam premix freagairt PCR àrd-èifeachdais | 1ml | DP-20031 | -20 ℃ | |
| 3 × 1.7 ml | DP-20032 | |||
| 3 × 1.7 ml | DP-20032-B | -20 ℃ | ||
| 50 ml | DP-20034-B | |||
| Siostam premix freagairt 2 × PCR Rapid 2 × PCR Mix | 1ml | DP-20041 | -20 ℃ | |
| 3 × 1.7 ml | DP-20042 | |||
| 500 × 20 μl rxns | QP-01012 | -20 ℃ | ||
| 2000 × 20 μl rxns | QP-01014 | |||
| 500 × 20 μl rxns | QP-01022 | -20 ℃ | ||
| 2000 × 20 μl rxns | QP-01024 | |||
| Sreath RT | 25 × 20μl Ath-bheachdan | RT-01011 | -20 ℃ | |
| 100 × 20μl Reaction | RT-01012 | |||
| (Master Premix airson synthesis cDNA ciad-sreath airson PCR Fìor-ùine) | 200 × 10μl Reaction | RT-01022 | -20 ℃ | |
| 800 × 10μl Reaction | RT-01023 | |||
| Master Premix airson synthesis cDNA a’ chiad sreath airson PCR Real Time le gDNase | 100 × 20μl Reaction | RT-01032 | -20 ℃ | |
| Laoch Lnc-RTTM I (Le gDNase)(Super Premix airson synthesis cDNA sa chiad sreath bho lncRNA) | 25 × 20μl Reaction | RTL-09098 | -20 ℃ | |
| 50 × 20μl Reaction | RTL-09099 | |||
| Sreath RT-PCR | 100 rxns | RT-02011 | -20 ℃ | |
| 500 rxns | RT-02012 | |||
| 100 rxns | RT-02021 | -20 ℃ | ||
| 500 rxns | RT-02022 | |||
| Sreath RT-qPCR | 100 rxns | RT-02111 | -20 ℃ | |
| 500 rxns | RT-02112 | |||
| 100 rxns | RT-02131 | -20 ℃ | ||
| 500 rxns | RT-02132 | |||
| Comharra DNA | 50 Ullamh | DL-20011 | 4 ℃ | |
| 200 Preps | DL-20012 | |||
| 50 Ullamh | DL-30011 | 4 ℃ | ||
| 200 Preps | DL-30012 |
